Evidence for and expression of a laccase gene in three basidiomycetes degrading humic acids |
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Authors: | T Scheel U Hölker S Ludwig M Höfer |
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Institution: | Botanisches Institut der Universit?t Bonn, Kirschallee 1, D-53115 Bonn, Germany Tel.: +49-228-735545 Fax: +49-228-735504, DE
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Abstract: | The majority of lignin-degrading basidiomycetes are able to depolymerize humic acids. In this presentation the relationship
and possible similarities between enzymes involved in lignin degradation and humic acid depolymerization were examined on
the genetic level. We have cloned fragments of the gene encoding the extracellular ligninolytic enzyme laccase from Clitocybula dusenii, Nematoloma frowardii and a fungal strain designated i63-2, and compared the three sequences with those of several other published laccase genes.
The sequenced fragments displayed a high homology both on the DNA (97%–77%) and amino acid (100%–85%) level. Furthermore,
the expression of this gene in the above-mentioned fungi was demonstrated by a nested polymerase chain reaction with cDNA
as template.
Received: 3 February 1998 / Received revision: 31 August 1998 / Accepted: 3 September 1998 |
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