A GABA(A) receptor of defined subunit composition and positioning: concatenation of five subunits |
| |
Authors: | Baur Roland Minier Frédéric Sigel Erwin |
| |
Affiliation: | Department of Pharmacology, University of Bern, Friedbühlstrasse 49, CH-3010 Bern, Switzerland. |
| |
Abstract: | We show that the five subunits of a gamma-aminobutyric acid type A receptor (GABA(A) receptor) can be concatenated to yield a functional receptor. This concatenated receptor alpha(1)-beta(2)-alpha(1)-gamma(2)-beta(2) has the advantage of a known subunit arrangement. Most of its functional properties are not significantly different from a receptor formed by individual subunits. Extent of expression amounted to about 40% of that of non-concatenated receptors in Xenopus oocytes, after injection of oocytes with comparable amounts of cRNA coding for concatenated and non-concatenated receptors. The ability to express receptors consisting of five subunits enables detailed studies of GABA(A) receptor subtype selective compounds. |
| |
Keywords: | GABA, γ-aminobutyric acid GABAA receptor, γ-aminobutyric acid type A receptor |
本文献已被 ScienceDirect PubMed 等数据库收录! |