Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome |
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| Authors: | Thorsten Stellberger Roman Häuser Armin Baiker Venkata R Pothineni Jürgen Haas Peter Uetz |
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| Institution: | 1.Institute of Toxicology and Genetics,Karlsruhe Institute of Technology,Karlsruhe,Germany;2.Max-von-Pettenkofer Institute,Ludwig-Maximilians-University of Munich,München,Germany;3.Division of Pathway Medicine,University of Edinburgh,Crescent,UK;4.J Craig Venter Institute (JCVI),9704 Medical Center Drive,Rockville,USA |
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| Abstract: | Background Yeast two-hybrid (Y2H) screens have been among the most powerful methods to detect and analyze protein-protein interactions.
However, they suffer from a significant degree of false negatives, i.e. true interactions that are not detected, and to a
certain degree from false positives, i.e. interactions that appear to take place only in the context of the Y2H assay. While
the fraction of false positives remains difficult to estimate, the fraction of false negatives in typical Y2H screens is on
the order of 70-90%. Here we present novel Y2H vectors that significantly decrease the number of false negatives and help
to mitigate the false positive problem. |
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