New method for selection of hydrogen peroxide adapted bifidobacteria cells using continuous culture and immobilized cell technology |
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| Authors: | Valeria Mozzetti Franck Grattepanche Déborah Moine Bernard Berger Enea Rezzonico Leo Meile Fabrizio Arigoni Christophe Lacroix |
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| Institution: | 1.Laboratory of Food Biotechnology,Institute of Food Science and Nutrition,Zürich,Switzerland;2.Nestlé Research Center,Vers-chez-les-Blanc,Switzerland |
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| Abstract: | Background Oxidative stress can severely compromise viability of bifidobacteria. Exposure of Bifidobacterium cells to oxygen causes accumulation of reactive oxygen species, mainly hydrogen peroxide, leading to cell death. In this
study, we tested the suitability of continuous culture under increasing selective pressure combined with immobilized cell
technology for the selection of hydrogen peroxide adapted Bifidobacterium cells. Cells of B. longum NCC2705 were immobilized in gellan-xanthan gum gel beads and used to continuously ferment MRS medium containing increasing
concentration of H2O2 from 0 to 130 ppm. |
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