Highly restricted expression of Cre recombinase in cerebellar Purkinje cells |
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Authors: | Zhang Xin-Mei Ng Alam Hoi-Lam Tanner Julian A Wu Wu-Tian Copeland Neal G Jenkins Nancy A Huang Jian-Dong |
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Institution: | Department of Biochemistry, University of Hong Kong, Faculty of Medicine Building, Pokfulam, Hong Kong SAR, China. |
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Abstract: | The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. |
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Keywords: | Pcp2 L7 Cre recombinase BAC transgenic mouse cerebellum Purkinje cells |
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