乙醇对着床前小鼠胚胎体外发育的影响

用含不同浓度乙醇的Whitten氏培养液对小鼠2细胞、4细胞、8细胞和桑椹期胚胎分别进行体外培养，研究了乙醇对小鼠不同发育时期胚胎体外发育的影响。首先利用含0、0．1％、0．5％、1．0％、1．5％、2．0％、3．0％、5．0％和10．0％乙醇的Whitten氏培养液对2细胞胚胎进行培养，发现小鼠2细胞胚胎对培养液中乙醇浓度的耐受极限在1．5％左右。然后又用含1％和3％乙醇的Whitten氏培养液分别对小鼠2细胞、4细胞、8细胞和桑椹期胚胎进行培养。结果发现：含1％乙醇的培养液对于8细胞胚胎和桑椹胚的囊胚形成有促进作用，而在2细胞和4细胞胚胎中则影响不明显。3％乙醇则对各期胚胎均有不同程度的抑制作用，但随着胚胎发育其对乙醇的耐受力逐渐增强。

EFFECTS OF ETHANOL ON THE DEVELOPMENT OF MOUSE PREIMPLANTATION EMBRYOS IN VITRO

Mouse embryos at the stage of 2 cell, 4 cell, 8 cell and morula were cultured in Whitten's medium to study the effects of ethanol on the developmental embryo. Tolerance threshold of 2 cell mouse embryos to ethanol was around 1 5% when these embryos were cultured in Whitten's medium with 0, 0 1%,0 5%,1 0%, 1 5%, 2 0%, 3 0%, 5 0% and 10 0% ethanol, respectively. Then the mouse embryos at the stage of 2 cell, 4 cell, 8 cell and morula were cultured in Whitten's medium with 1 0% and 3 0% ethanol to see the sensitivity of mouse embryos to ethanol. The results suggested that 1 0% ethanol appeared to have beneficial effects on the cavitation of 8 cell embryos and morula. However, the effects of 1 0% ethanol on 2 cell and 4 cell embryos were not so obviously. Embryonic development at the stage of 2 cell, 4 cell, 8 cell and morula was more or less inhibited by 3 0% ethanol. Tolerance of embryos to ethanol becomes stronger in late developmental stages of the embryo.