A case of mutagen specificity attributable to a plating medium effect |
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Authors: | C H Clarke |
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Institution: | (1) M.R.C. Mutagenesis Research Unit, Institute of Animal Genetics, West Mains Road, Edinburgh 9, Schottland |
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Abstract: | Summary In anadn
–
met
– di-auxotrophic strain ofSchizosaccharomyces pombe met
+ reversions are several hundred times more frequent thanadn
+ reversions after treatment with ultra-violet light. They are only slightly more frequent thanadn
+ reversions when HNO2 is used as a mutagen ( mutagen specificity ). The poor response of theadn-1,199 allele to the mutagenic action of U.V. can be largely overcome by replacing themet-4,D19 allele with its normalmet
+ allele (influence of the genetic background). It was shown that both the mutagen specificity and its dependence on the genetic background are due, largely at least, to the inhibition ofadn
+ reversions on a plating medium containingl-methionine. This inhibition is very strong for U.V.-induced reversions but only weak for HNO2-induced ones. It would be wrong to assume that other mutants at theadenine-1 locus behave in the same manner.With 1 Figure in the Text |
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Keywords: | |
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