Exo- and endo-glucanolytic activity of cellulases purified from Trichoderma reesei |
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| Authors: | F.M. Gama M. Vilanova M. Mota |
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| Affiliation: | (1) Centro de Engenharia Biológica - IBQF, Universidade do Minho, Braga, Portugal;(2) Laboratório de Imunologia, Largo do Prof., Instituto de Ciências Biomédicas de Abel Salazar, Abel Salazar, Porto, Portugal |
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| Abstract: | Four cellulases, produced by Trichoderma reesei, have been purified by preparative isoelectric focusing (Rotofor), size exclusion (Sephacryl 100 HR), anionic (Mono Q) and cationic (Mono S) chromatography and chromatofocusing (Mono P). Enzymatic activity with a large number of substrates allowed the proteins to be classified as: cellobiohydrolase I, cellobiohydrolase II, endoglucanase I and endoglucanase II. The exo- or endo-glucanase character of these enzymes was analysed by using a technique based on the measurement of the Avicel insoluble fibres reducing power. © Rapid Science Ltd. 1998 |
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