Phosphatidylinositol-specific phospholipase D: a pathway for generation of a second messenger |
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| Authors: | J Balsinde E Diez F Mollinedo |
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| Affiliation: | Unidad de Biomembranas Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain. |
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| Abstract: | We have found a phospholipase D activity in the postnuclear fraction of human neutrophils which is stimulated by incubation of cells with the calcium ionophore A23187. The phospholipase D activity was assessed by both phosphatidate formation and free inositol release from phosphatidylinositol substrate. The phospholipase D activity shows an optimum pH of 7.5 and hydrolyzes specifically phosphatidylinositol. These results suggest that this phosphatidylinositol-specific phospholipase D can play a role in cell activating process. |
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