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Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion |
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| Authors: | Sandra Austin Maria A Baer John P Helgeson |
| Institution: | 1. Departmentof Plant Pathology, University of Wisconsin, Madison, WI 53706 U.S.A.;2. UDSA, ARS, NCR, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706 U.S.A. |
| Abstract: | Tetraploid somatic hybrids were produced by protoplast fusion between Solanum brevidens, a diploid non-tuber-bearing wild species and a diploid tuber-bearing potato line derived from an S. tuberosum Gp. Phureja-Stenotumum population. S. brevidens has resistance to potato leaf roll virus (PLRV) and frost but is difficult to cross sexually with cultivated potato. Hybridity was verified by morphological characteristics and cytological observations. Nine of ten hybrids tested showed resistance to PLRV. Hybrids produced fertile pollen and eggs which may allow beneficial traits of S. brevidens to be incorporated into a conventional potato breeding programme. |
| Keywords: | Somatic fusion transfer of resistance potato leaf roll virus potato Cul culture Dif differentiation gibberellic acid IAA indoleacetic acid MS Murashige and Skoog PEG polyethylene glycol PLRV potato leaf roll virus |
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