Crown galls induced by octopine-degrading biotype 3 strains of Agrobacterium tumefaciens contain a new form of lysopine dehydrogenase |
| |
Authors: | L. Otten E. Szegedi |
| |
Affiliation: | 1. Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30 F.R.G.;2. University of Horticulture, Research Institute of Viticulture and Enology, Kecskemet, P.O. Box 25, 6000 Hungary |
| |
Abstract: | Extracts of crown gall tumours induced on Kalanchoe daigremontiana by the biotype 3 strains AB-3, AT-6 and Hm-1 were compared with extracts of tumours induced by the wide host range octopine strain B6S3 for their ability to convert lysine, arginine, histidine and methionine into the corresponding opines: lysopine, octopine, histopine and methiopine. In contrast to B6S3 tumour extracts which readily converted all precursors into opines, extracts of tumour tissue induced by biotype 3 strains produced mainly lysopine and octopine. It can therefore be concluded that the enzymes found in tumours induced by AB-3, AT-6 and Hm-1 are different from the well-known lysopine dehydrogenase from B6S3 tumours. |
| |
Keywords: | agrobacterium crown gall opines LpDH lysopine dehydrogenase MES OCS octopine synthase |
本文献已被 ScienceDirect 等数据库收录! |
|