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Regulation of nitrate reductase in suspension culture of Silene alba Immunochemical approach
Authors:Michel Poulle  Serge Rambour
Affiliation:Laboratoire de Physiologie végétale, Université des Sciences et Techniques de Lille 1, 59655 Villeneuve D''Asq Cedex France
Abstract:Silene alba cells grown on nitrate, usually develop NADH-nitrate reductase activity only at the beginning of their growth cycle. Immunodiffusion assays, with a specific nitrate reductase antiserum, revealed the presence of cross-reacting material in cells harvested at any time during their culture. Cells grown on ammonium lacked NADH-nitrate reductase activity but contained cross-reacting material. It is suggested that S. alba cells contain an enzymically inactive, antigenic form of nitrate reductase regardless of the nitrogen source.
Keywords:nitrate reductase regulation  immunochemical approach  cross-reaction  MES  NR  nitrate reductase
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