Regulation of nitrate reductase in suspension culture of Silene alba Immunochemical approach |
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Authors: | Michel Poulle Serge Rambour |
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Affiliation: | Laboratoire de Physiologie végétale, Université des Sciences et Techniques de Lille 1, 59655 Villeneuve D''Asq Cedex France |
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Abstract: | Silene alba cells grown on nitrate, usually develop NADH-nitrate reductase activity only at the beginning of their growth cycle. Immunodiffusion assays, with a specific nitrate reductase antiserum, revealed the presence of cross-reacting material in cells harvested at any time during their culture. Cells grown on ammonium lacked NADH-nitrate reductase activity but contained cross-reacting material. It is suggested that S. alba cells contain an enzymically inactive, antigenic form of nitrate reductase regardless of the nitrogen source. |
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Keywords: | nitrate reductase regulation immunochemical approach cross-reaction MES NR nitrate reductase |
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