Characterization of the cytoplasmic fibrils of Treponema refringens (Nichols) |
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Authors: | Susan R Eipert S H Black |
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Institution: | (1) Department of Microbiology and Public Health, Michigan State University, 48824 East Lansing, Michigan, USA;(2) Present address: Department of Medical Microbiology and Immunology, College of Medicine, Texas A & M University, 77843 College Station, TX, USA |
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Abstract: | The cytoplasmic fibrils of Treponema refringens were studied in situ by electron microscopy of thin sectioned and negatively stained cells. From 5 to 21 parallel fibrils ran through the cell in a band adjacent to the inner side of the cytoplasmic membrane, on the inner sides of the curves of the spirochete. The nuclear areas of cells were adjacent to the fibrils. Cross sections of fibrils isolated from cells which had been lysed were polygonal and not uniformly electron dense. Polyacrylamide gel electrophoresis of partially purified fibril preparations indicated their main component to be a protein with a molecular weight of 97,000. Fibrils were solubilized by 1% trypsin, 1% pronase, 6 M urea, 1 N HCl, 0.005 N NaOH or 1.3% sodium dodecyl sulfate. By electron microscopy of negatively stained isolated fibrils, each fibril was found to be a complex arrangement of strands rather than a single tubule.Abbreviations CM
Cytoplasmic membrane
- PTA
Phosphotungstic acid
- UOx
Uranyl oxalate
- SDS
sodium dodecyl sulfate
This communication is Journal Acticle No. 7644 from the Michigan Agricultural Experiment Station |
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Keywords: | Cytoplasmic fibrils Treponemes Treponema refringens Fine structure Cell lysis |
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