水痘-带状疱疹病毒地方分离株在兔脑神经细胞中的形态与形态发生特征

为阐明水痘-带状疱疹病毒济南分离株（VZVJ1）在兔脑神经细胞（RNC）中的形态与形态发生特征，我们利用超薄切片电子显微镜技术对感染VZVJ1的RNC进行了观察研究。结果表明RNC在感染VZVJI6h后核内可见散在的核衣壳，12h后细胞核和细胞质内核衣壳明显增多，24h达高峰，而细胞核和细胞质内的成熟病毒颗粒较少见。病毒大小、形态基本一致，呈圆形或椭圆形，核心直径30～50nm，核衣壳74～96nm，成熟病毒110～180nm。核衣壳内有3种类型的核心，即电子致密核心、部分致密核心和电子透明核心。细胞核和细胞质内均可见核心样电子致密体和布纹样结构。在细胞质内还可见少量“繁殖复合体”，由膜性结构包绕多个囊泡构成。提示VZVJ1在RNC中的形态发生不同于其它性质的细胞。

Morphology and Morphogenesis of the Isolated Varicella-Zoster Virus in Rabbit Brain Nerve Cell

To Investigate the morphology and morphogenesis of the isolated varicella-zoster virus (VZV) in rabbit brain nerve cell (RNC), ultrathin section electron microscopy was used for study. A small number of nucleocapsids were observed in the nuclei of RNC at 6 h post infection. A large number of necleocapsids were seen in the nuclei and cytoplasm at 12 h post infection, and the peak was at 24 h, but enveloped virions were rarely seen in the nuclei and cytoplasm. The virions were spherical. Cores, necleocapsids and enveloped virions are 30～50 nm, 74～96 nm and 110～180 nm in diameter respectively. There were three kinds of nucleocapsids with electron dense, particle and empty cores. Electron dense body-like core and plate structure were observed both in nuclei and cytoplasm. “Replication complexes” composed of vacuoles enveloped by membrane was seen in the cytoplasm. These results indicated that VZVJ1 showed different morphogenesis in RNC and other cells.