Microplate assay of elevated esterase activity in individual pyrethroid-resistant mosquitoes |
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| Authors: | Arunima Sahgal Sarita Kumar M K K Pillai |
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| Institution: | (1) Department of Zoology, University of Delhi, 110 007 Delhi, India |
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| Abstract: | Involvement of esterase-mediated hydrolysis as a mechanism of pyrethroidresistance in three species of mosquitoes,viz., Aedes aegypti, Culex quinquefasciatus andAnopheles Stephensi was investigated by microplate assay of Β-esterases in individual larva and adult female and male mosquitoes. Assuming an
absorbance value of 0.4 and above at 555 nm as the threshold level of elevated esterase activity which confers resistance,
frequency distributions of such individual test mosquitoes were constructed in resistant and susceptible populations. The
results indicate the involvement of ester hydrolysis of Pyrethroids as a predominant mechanism of pyrethroid-resistance in
the larvae ofCulex quinquefasciatus but not inAedes aegypti. However, a marginal role of esterases is indicated in the larvae ofAnopheles stephensi. |
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| Keywords: | Non-specific esterases pyrethroid-resistance microplate assay Aedes aegypti Culex quinquefasciatus Anopheles stephensi |
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