Cell-free synthesis of putative neurophysin precursors from rat and mouse hypothalamic poly (A)-RNA. |
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Authors: | C Lin P Joseph-Bravo T Sherman L Chan J F McKelvy |
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Affiliation: | 1. Department of Biochemistry, University of Texas Health Science Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235 USA;2. Department of Cell Biology, Baylor College of Medicine, Houston, Texas, USA;3. Department of Medicine, Baylor College of Medicine, Houston, Texas USA |
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Abstract: | Poly(A)-containing RNA has been isolated from rat and mouse hypothalamic tissue and used to direct the synthesis of polypeptides in cell-free systems derived from wheat germ extract and rabbit reticulocyte lysate in the presence of [35S]-L-cysteine and [3H]-L-proline. Translation products were subjected to immunoprecipitation using an antiserum to rat neurophysin proteins. Following purification of the immunoprecipitates by protein A-Sepharose chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single polypeptide species of molecular weight 17,500 derived from both cell-free systems. |
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