Enrichment and isolation of nitrogen fixing hydrogen bacteria |
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Authors: | J. Wiegel H. G. Schlegel |
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Affiliation: | (1) Institut für Mikrobiologie der Gesellschaft für Strahlen- und Umweltforschung mbH, München, in Göttingen, Grisebachstr. 8, D-3400 Göttingen, Germany |
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Abstract: | An enrichment method for nitrogen fixing hydrogen bacteria is described. The procedure invariably resulted in the isolation of yellow-pigmented coryneform bacterial strains assigned to Corynebacterium autotrophicum. The procedure included a serial transfer in an ammonium-free mineral liquid medium under an atmosphere of 10% hydrogen, 5% oxygen, 10% carbon dioxide and 75% nitrogen, followed by a short alkali treatment and by streaking on nutrient broth-succinate agar. The ability to fix nitrogen was confirmed by the acetylene reduction test and by 15N2 incorporation. |
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Keywords: | Hydrogen bacteria Corynebacterium autotrophicum N2-Fixation 15N2-Incorporation Specific enrichment culture Acetylene reduction Coryneform bacteria |
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