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Umbilical cord blood processing using Prepacyte-CB increases haematopoietic progenitor cell availability over conventional Hetastarch separation |
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| Authors: | C Basford N Forraz† S Habibollah† K Hanger C P McGuckin† |
| Institution: | Newcastle Centre for Cord Blood, Institute of Human Genetics, Newcastle University, Newcastle upon Tyne, UK;and CTI-LYON, Cell Therapy research Institute, Parc Technologique de Lyon-St Priest, Saint Priest Lyon, France |
| Abstract: | Background: Currently the most frequently used method for umbilical cord blood separation in many hospitals across the UK and the rest of the world, where small-to-medium amounts of samples are processed, is Hetastarch, a mechanical, starch-based method, which causes red cell agglutination by rouleaux formation. Aim: In this study, a novel method (Prepa-Cyte-CB), in comparison with Hetastarch as part of an FDA-approved clinical study, was evaluated. Materials and Methods: Validation of data included recovery of nucleated and CD34+ cells, red blood cell reduction, colony forming unit potential, flow cytometric analysis and sterility tests. Results: PrepaCyte-CB, in comparison with Hetastarch offers fast, reliable separation with improved recovery of nucleated cells, 72.03% (±8.48 SD) compared to 58.09% (±20.06 SD), and CD34+ haematopoietic progenitor cells, 76% (±19.54 SD) compared to 64.19% (±29.77 SD). PrepaCyte-CB was also 12-fold more efficient in removing red blood cells and haemoglobin (P < 0.001) than Hetastarch. Conclusions: These results show that PrepaCyte-CB offers superior separation of UCB when compared to Hetastarch. |
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