Purification and partial characterization of phytotoxic glycopeptides secreted by Colletotrichum gloeosporioides |
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Authors: | M. Barjau D. Druet L. -C. Comeau |
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Affiliation: | (1) Laboratoire de Chimie Biologique Appliquée, U.D.E.S.A.M., 3 Place Victor-Hugo, 13331 Marseille Cedex 8, France |
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Abstract: | Colletotrichum gloeosporioides secretes a phytotoxic compound that causes anthracnose in Hevea brasiliensis: as a first step we attempted to follow mycelium growth, pH and toxin production by this fungus. The compound was isolated and partially purified by ultrafiltration, acetone precipitation and affinity and gel-permeation chromatography. Material purified was largely carbohydrate with a small protein fraction. Galactose, mannose and rhamnose were the neutral sugars encountered, and serine and threonine the major amino acids found. We propose a molecular mass for this substance of 50333 Da. |
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