Novel candidate substrates of AMP-activated protein kinase identified in red blood cell lysates |
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| Authors: | Ramon F. Thali |
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| Affiliation: | a Institute of Cell Biology, ETH Zurich, Zurich, Switzerland
b Functional Genomics Center Zurich (FGCZ), University of Zurich, Zurich, Switzerland |
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| Abstract: | AMPK is a metabolic stress-sensing kinase with important functions for red blood cell (RBC) survival. By using a proteomic approach, we identified putative AMPK targets in hemoglobin-depleted lysates of RBC, including metabolic enzymes, cytoskeletal proteins and enzymes involved in the oxidative stress response. These data tie in with the phenotypic observations of AMPKα1-deficient RBC and provide reference for future studies. |
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| Keywords: | 2-DE, two-dimensional electrophoresis AMPK, AMP-activated protein kinase DTT, dithiothreitol MudSeeK, multidimensional substrate-screen for protein kinases RBC, red blood cells |
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