Adaptor protein GULP is involved in stabilin-1-mediated phagocytosis |
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Authors: | Seung-Yoon Park Kae-Bok Kang |
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Institution: | a Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea b Department of Biochemistry, School of Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea |
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Abstract: | The clearance of apoptotic cells is critical during cellular homeostasis as well as inflammation resolution. Recently, we found that stabilin-1 is a phagocytic receptor that is involved in the clearance of apoptotic cells. However, the downstream signaling pathway of stabilin-1-mediated phagocytosis remains to be investigated. Here we identify that GULP is able to specifically interact with the NPxF/Y motif of stabilin-1 cytoplasmic region. The PTB domain of GULP is necessary for interaction with stabilin-1. GULP is enriched around PS-coated beads for phagocytosis and co-localized with stabilin-1. Downregulation of endogenous GULP expression decreased stabilin-1-mediated phagocytosis. Thus, these results indicate that GULP functions as an adaptor protein for stabilin-1-mediated phagocytosis. |
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Keywords: | GULP Stabilin-1 Phosphatidylserine Phagocytosis Aged RBC |
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