Gene expression profiling in porcine mammary gland during lactation and identification of breed-and developmental-stage-specific genes |
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Authors: | SU Zhixi DONG Xinjiao ZHANG Bing ZENG Yanwu FU Yan YU Jun HU Songnian |
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Institution: | 1. James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008, China 2. School of Life and Environmental Sciences, Wenzhou Normal College, Wenzhou 325027, China 3. James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008, China;Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China |
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Abstract: | A total of 28941 ESTs were sequenced from five 5′-directed non-normalized cDNA libraries, which were assembled into 2212 contigs
and 5642 singlets using CAP3. These sequences were annotated and clustered into 6857 unique genes, 2072 of which having no
functional annotations were considered as novel genes. These genes were further classified into Gene Ontology categories.
By comparing the expression profiles, we identified some breed-and developmental-stage-specific gene groups. These genes may
be relative to reproductive performance or play important roles in milk synthesis, secretion and mammary involution. The unknown
EST sequences and expression profiles at different developmental stages and breeds are very important resources for further
research. |
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Keywords: | expressed sequence tags expression profile domestic pig mammary gland |
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