Growth and hepatospecific gene expression of human hepatoma cells in a defined medium |
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Authors: | G J Darlington J H Kelly G J Buffone |
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Institution: | (1) Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Texas Children's Hospital, 6621 Fannin Street, 77030 Houston, TX |
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Abstract: | Summary The production of albumin, α-fetoprotein (AFP), and α-1 antitrypsin has been compared among human hepatoma cells cultured
in medium containing serum, medium containing hormones and growth factors, and a basal medium containing selenium as the only
supplement. Growth is sustained in all three media, and the expression of all three proteins was maintained for over 4 mo.
in the various media. However, the quantitative production of albumin and AFP were dramatically different in the three media.
Two hormones, insulin and triiodothyronine, influenced the level of secreted proteins. Triiodothyronine increases the amount
of secreted albumin whereas insulin at 10 μg/ml reduced the level of total secreted protein. |
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Keywords: | hepatoma albumin α -fetoprotein α 1 antitrypsin defined medium |
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