Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein |
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| Authors: | Eiji Yokoyama Katsumi Doi Makoto Kimura Seiya Ogata |
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| Affiliation: | Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-81, Japan; Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-81, Japan |
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| Abstract: | Abstract Streptomyces plasmid pSA1.1 accumulated single-stranded DNA as replication intermediates in S. lividans ; therefore, this plasmid was considered to replicate by a rolling-circle mechanism. A DNA-binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA-Cellulose column, then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein has a high homology with bacterial histone-like proteins. In the gel retardation assay, this protein bound with the single-stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1. |
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| Keywords: | DNA-binding protein Histone-like protein Plasmid Rolling-circle replication Streptomyces |
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