Estimation of the number of full sibling families at an oviposition site using RAPD-PCR markers: applications to the mosquito Aedes aegypti |
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| Authors: | B L Apostol W C Black IV B R Miller P Reiter B J Beaty |
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| Institution: | (1) Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, 80523 Fort Collins, CO, USA;(2) Medical Entomology and Ecology Branch, Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, P.O. Box 2087, 80522 Fort Collins, CO, USA;(3) Dengue Branch, Division of Vector-Borne Infectious Diseases, Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service ,U.S. Department of Health and Human Services, 2 Calle Casia, 00921-3200 San Juan, Puerto Rico |
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| Abstract: | There are many species in which groups of individuals encountered in the field are known to consist of mixtures of full-sibling families. We describe a statistical technique, based on the use of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers, that allows for the estimation of the number of families contained in these groups. We test the technique on full-sibling families of the mosquito Aedes aegypti, a species that distributes its eggs among several locations. Mixtures of 10 families with 15 individuals per family were analyzed using 40 RAPD-PCR loci amplified by 5 primers. Our analysis accurately estimated the number of families. The technique was accurate when the number of families was small or when family sizes were small and variable. |
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| Keywords: | RAPD-PCR DNA Fingerprinting Sibling analysis Oviposition Aedes aegypti |
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