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Development of glucose biosensor based on reconstitution of glucose oxidase onto polymeric redox mediator coated pencil graphite electrodes
Affiliation:1. Materials Science Centre, Department of Nuclear Physics, University of Madras, Guindy Campus, Chennai 600025, India;2. Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai 600025, India;1. Bioénergétique et Ingénierie des Protéines, UMR 7281, CNRS-AMU, 31 Chemin Aiguier, 13009 Marseille, France;2. Institut de Sciences des Matériaux de Mulhouse, UMR 7361, CNRS, 15 rue Starcky, 68057 Mulhouse, France;3. Centre de Recherche Paul Pascal, UPR 8641, CNRS, Bordeaux University, 33600 Pessac, France
Abstract:In this study, a novel glucose biosensor was fabricated by reconstitutional immobilization of glucose oxidase (GOx) onto a poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)) film coated pencil graphite electrode (PGE). The amperometric current response of poly(GMA-co-VFc)-GOx to glucose is linear in the concentration range between 1 and 16 mM (correlation coefficient of 0.9998) with a detection limit of 2.7 μM (S/N = 3). Experimental parameters were studied in detail and optimized, including the pH and temperature governing the analytical performance of the biosensor. The stability and reusability of the biosensor as well as its kinetic parameters have also been studied.
Keywords:Glucose  Mediator  Ferrocene  Amperometric biosensor
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