Scaling-up the synthesis of myristate glucose ester catalyzed by a CALB-displaying Pichia pastoris whole-cell biocatalyst |
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| Institution: | 1. Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China;2. Pharmacy School, Wenzhou Medical University, Wenzhou 325035, PR China;1. Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, China;2. Huaian Institute of Supervision & Inspection on Product Quality, Huaian 223300, China;3. Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi 214122, China;1. Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China;2. Wuhan Center for Magnetic Resonance, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, PR China;1. Department of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Silesian University of Technology, B. Krzywoustego 4, 44-100 Gliwice, Poland;2. Department of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, Borowska 211A, 50-368 Wroc?aw, Poland;3. Systems Engineering Group, Institute of Automatic Control, Silesian University of Technology, B. Krzywoustego 8, 44-100 Gliwice, Poland;1. N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, Lavrentjev Avenue 9, 630090 Novosibirsk, Russia;2. Novosibirsk State University, Pirogova St. 2, 630090 Novosibirsk, Russia;1. Institute of Food Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China;2. Research Institute of Food and Biotechnology, SPC Group, Seoul 08826, Republic of Korea;3. Department of Foodservice Management and Nutrition, Sangmyung University, Seoul 03016, Republic of Korea;4. Biotechnology Center of Ho Chi Minh City, 2374, Highway 1, Quarter 2, Trung My Tay Ward, District 12, Ho Chi Minh City, Viet Nam;5. Department of Food Technology, An Giang University, An Giang 076, Viet Nam;6. Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea;7. Food Technology Department, Saigon Technology University, 180 Cao Lo, Ward 4, Dist. 8, HCM City, Viet Nam;8. School of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea |
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| Abstract: | The novel whole-cell biocatalyst Candida antarctica lipase B displaying-Pichia pastoris (Pp-CALB) is characterized by its low preparation cost and could be an alternative to the commercial immobilized Candida antarctica lipase B (CALB). This study addresses the feasibility of using Pp-CALB in large scale glucose fatty acid esters production. 1,2-O-Isopropylidene-α-d-glucofuranose (IpGlc) was used as the acyl acceptor to overcome the low solubility of glucose in an organic solvent and to avoid the addition of toxic co-solvents. IpGlc significantly improved the Pp-CALB catalyzing esterification efficiency when using long chain fatty acids as the acyl donor. Under the preferred operating conditions (50 °C, 40 g/L molecular sieve dosage and 200 rpm mixing intensity), 60.5% of IpGlc converted to 6-O-myristate-1, 2-O-isopropylidene-α-d-glucofuranose (C14-IpGlc) after a 96-h reaction in a 2-L stirred reactor. In a 5-L pilot scale test, Pp-CALB also showed a similar substrate conversion rate of 55.4% and excellent operational stability. After C14-IpGlc was collected, 70% trifluoroacetic acid was adopted to hydrolyze C14-IpGlc to myristate glucose ester (C14-Glc) with a high yield of 95.3%. In conclusion, Pp-CALB is a powerful biocatalyst available for industrial synthesis, and this study describes an applicable and economical process for the large scale production of myristate glucose ester. |
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| Keywords: | Yeast whole cell biocatalyst Myristate glucose ester Scale synthesis |
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