Evaluation of three individual glucosyltransferases produced by Streptococcus mutans using monoclonal antibodies |
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Authors: | Yukihide Tomita Xia Zhu Kuniyasu Ochiai Yasuji Namiki Tamami Okada Takuji Ikemi Kazuo Fukushima |
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Institution: | Department of Microbiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271, Japan; Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271, Japan |
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Abstract: | Abstract We previously established murine hybridomas producing a monoclonal antibody monospecific against three glucosyl-transferases (I, SI and S) of Streptococcus mutans which contribute to dental caries formation. Here, we developed a new immunochemical technique (cross-dot system) with which individual levels of glucosyltransferases expressed by S. mutans can be evaluated. We also examined glucosyltransferase production and in vitro artificial plaque formation by a reference strain and several clinical isolates of S. mutans . The findings indicate that the levels of glucosyltransferases produced greatly vary with the cells and the culture medium, and that the cells producing high levels of both glucosyltransferase-SI and glucosyltransferase-I enzymes may possess high in vitro artificial plaque forming ability. We suggest that the cross-dot system will be useful for estimating the cariogenic potential of S. mutans isolates. |
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Keywords: | Streptococcus mutans Monoclonal antibody Immuno-dot assay |
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