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Advances in alternative DNA delivery techniques |
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| Authors: | D D Songstad D A Somers R J Griesbach |
| Institution: | (1) Pioneer Hi-Bred International, 7300 NW 62nd Ave., P.O. Box 1004, 50131 Johnston, Iowa, USA;(2) Department of Agronomy and Plant Genetics, Plant Molecular Genetics Institute, University of Minnesota, 411 Borlaug Hall, 1991 Buford Circle, 55108 St. Paul, MN, USA;(3) Florist and Nursery Plant Research, U.S. National Arboretum, USDA/ARS, Barc-W, 20705 Beltsville, MD, USA |
| Abstract: | This review describes recent advances in alternative DNA-delivery techniques with particular emphasis on silicon carbide fibers, intact tissue electroporation, electrophoresis and microinjection. The advantages/disadvantages of each method along with a historical overview and theory of practice are discussed. |
| Keywords: | electrophoresis intact tissue electroporation microinjection silicon carbide fiber transformation |
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