Utilization ofEscherichia coli tac promoter inBacillus subtilis |
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Authors: | Baek Rak Lee M. Y. Pack |
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Affiliation: | (1) Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Cheongryang, P. O. Box 150, 130-650 Seoul, Korea |
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Abstract: | Summary The tac promoter originally constructed for the use inEscherichiacoli was fused to an endoglucanase structural gene isolated fromBacillussubtilis and the expression of the chimeric gene inB.subtilis was observed. The tac promoter-controlled gene expressed well inB.subtilis and produced endoglucanase during the exponential growth phase. |
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