|
|||||
|
|
The ITS region as a taxonomic discriminator between Fusarium verticillioides and Fusarium proliferatum |
|
| Authors: | I. Visentin D. Valentino P. Karlovsky F. Cardinale |
| Affiliation: | a DiVaPRA, Plant Pathology, University of Turin, via L. da Vinci 44, 10095 Grugliasco (TO), Italy b DiVaPRA, Plant Genetics and Breeding, University of Turin, via L. da Vinci 44, 10095 Grugliasco (TO), Italy c Molecular Phytopathology and Mycotoxin Research Unit, University of Göttingen, Grisebachstrasse 6, D-37077 Göttingen, Germany d ISPA, National Research Council, Via Amendola, 122/O - 70126 Bari (BA), Italy e Dept. of Arboriculture, University of Turin, via L. da Vinci 44, 10095 Grugliasco (TO), Italy |
| Abstract: | The maize pathogens Fusarium verticillioides (Fv) and Fusarium proliferatum (Fp) are morphologically very similar to one another, so Fp isolates have been often mistaken as Fusarium moniliforme (the former name of Fv). The only presently accepted morphological discriminator between these species is the presence/absence of polyphialides. Here, a collection of 100 Fusarium strains, isolated from infected maize kernels on plants grown in north-western Italy, were assigned as Fv or Fp on the basis of the presence/absence of polyphialides. This classification was tested on a subset of isolates by sexual crosses, ITS and calmodulin sequencing and AFLP profiling. An ITS-RFLP assay was extended to the full collection and to a number of Fv and Fp isolates of different geographical origin and hosts. The ITS region is proposed as taxonomically informative for distinguishing between Fp and Fv. |
| Keywords: | Fusarium proliferatum Fusarium verticillioides ITS Maize PCR |
| 本文献已被 ScienceDirect 等数据库收录！ | |