Nucleotide sequence identity of mitochondrial DNA from different human tissues |
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| Authors: | R J Monnat D T Reay |
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| Affiliation: | 1. Department of Medicine, Michigan State University, East Lansing, Michigan;2. Division of cardiovascular diseases, Department of Medicine, Lenox Hill Hospital, New York City, New York;3. Division of Cardiovascular diseases, Mount Sinai St Luke''s Roosevelt, New York City, New York;4. Department of Medicine, Staten Island University Hospital, Staten Island, New York City, New York;5. Division of Cardiology, New York Medical College at Westchester Medical Center, Valhalla, New York;6. Division of Cardiovascular diseases, Department of Medicine, St. John Hospital and Medical Center, Detroit, Michigan;7. Division of Cardiovascular diseases, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts;8. Division of Cardiovascular diseases, Icahn School of Medicine at Mt Sinai, New York City, New York;9. Division of Cardiovascular diseases, Henry Ford Health System, Detroit, Michigan;10. Brigham and Women''s Hospital Heart & Vascular Center, Harvard Medical School, Boston, Massachusetts;1. University of Arizona College of Medicine, Phoenix, Arizona;2. Wayne State University School of Medicine, Detroit, Michigan;3. Sri Venkateswara Medical College, Tirupati, India |
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| Abstract: | Recombinant DNA techniques have been used to search for mitochondrial (mt) nucleotide (nt) sequence differences between human tissues within an individual. mtDNA isolated from brain, heart, liver, kidney, and skeletal muscle of two different individuals was cleaved with SacI and XbaI, and then cloned in bacteriophage M13. Partial nt sequence determination of 121 independently isolated recombinant M13 clones containing either the cytochrome oxidase subunit III gene or the D-loop region of human mtDNA revealed base substitution differences between individuals, and between each individual and the published human mtDNA sequence. A majority of these base substitutions were transitions. No systematic nt sequence differences were identified between tissues within an individual, however. These results suggest that mtDNA sequence alterations do not accompany organogenesis, and that somatic mutations do not accumulate in the mtDNA of different human tissues to a level of greater than one nt substitution per molecule. |
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