Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3 |
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Authors: | Alexander S Arseniev Vladimir I Kondakov Vladimir N Maiorov Vladimir F Bystrov |
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Institution: | M.M. Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Ul.Vavilova-32, 117988, GSP-1, Moscow V-334, USSR |
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Abstract: | The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor. |
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Keywords: | NMR Nuclear Overhauser effect Peptide conformation Scorpion toxin |
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