Characterization and partial purification of an intestinal lipase. |
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Authors: | G Serrero R Négrel G Ailhaud |
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Institution: | Lister Institute of Preventive Medicine, Chelsea Bridge Road, London SW1W 8RH, U.K. |
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Abstract: | The isoelectric points of the blood group and gene-associated glycosyltransferases in human ovarian cyst fluids were found by isoelectric focusing to be in the pH range 9.5–10. The and transferases in serum had isoelectric points similar to those of the enzymes in cyst fluids but transferases in serum had considerably lower isoelectric points, in the pH range 6–7. The difference in the pI values of the and transferases in the serum of a donor of the genotype enabled the two enzymes to be preparatively separated by the isoelectric focusing technique. The dissimilarity in the pI values of the transferases in ovarian cyst fluids and serum samples indicates that the isoelectric point arises from a post-translational modification of the enzyme protein. |
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