Snail mRNA及其蛋白在乳腺癌中的表达研究

目的观察Snail蛋白及mRNA在人乳腺癌组织中的表达及其与临床病理特征的关系,并探讨它在乳腺癌发生、发展及转移中的作用。方法应用SP免疫组织化学和原位分子杂交方法检测Snail蛋白和Snail mRNA在70例乳腺浸润性导管癌、30例乳腺导管内癌、30例乳腺单纯性增生组织中的表达。结果①70例乳腺癌中,Snail蛋白和Snail mRNA阳性率分别为87.2%(61/70)、81.4%(57/70),分别高于乳腺导管内癌组织53.3%(16/30)、46.7%(14/30)和乳腺单纯性增生组织26.7%(8/30)、23.3%(7/30),三者相比有统计学意义(χ2=36.4,P<0.01;χ2=32.4,P<0.01)。②Snail蛋白和SnailmRNA在有淋巴结转移组中的阳性率为97.6%(40/41)、95.1%(39/41),无转移组阳性率为72.4%(21/29)、62.1%(18/29),两者相比有统计学意义(χ2=8.29,P<0.01);组织学分级Ⅲ级组明显高于Ⅰ、Ⅱ级组表达,但无统计学意义(χ2=0.72,P>0.05;χ2=0.98,P>0.05)。③Snail蛋白与Snail mRNA的表达与年龄、肿瘤大小均无关(P>0.05)。结论 Snail蛋白与Snail mRNA的表达呈正相关,与乳腺癌的发生发展、淋巴结转移密切相关,可作为判断乳腺癌预后、转移的生物学标志。

The expression of Snail mRNA and protein in breast cancer

Objective To observe the expression of Snail protein and mRNA in breast cancer and the correlationship between its expression and clinicopathological features,and to investigate its roles in the carcinogenesis and progression of breast cancer. Methods The expression of Snail protein and mRNA was detected by SP and in situ hybridization（ISH） in invasive ductal carcinoma（70 cases）,intraductal breast carcinoma（30 cases） and simplex breast hyperplasia（30 cases） respectively. Results ① In 70 cases of breast cancer,the expression rate of Snail protein and mRNA was 87.2%（61/70） and 81.4%（57/70） respectively,which was respectively higher than in intraductal breast carcinoma 53.3%（16/30）,46.7%（14/30） and simplex breast hyperplasia 26.7%（8/30）、23.3%（7/30）,with statistical significance（χ2=36.4,P0.01;χ2=32.4,P0.01）.② The expression rate of Snail protein and mRNA in the lymphatic metatasis group was 97.6%（40/41） and 95.1%（39/41）respectively,but 72.4%（21/29） and 62.1%（18/29） in the group without lymphatic metatasis respectively,also with statistical significance（χ2=8.29,P0.01）.The expression rate of Snail protein and mRNA in histopathological grade III was higher than that in histopathological grades II and I,but with no statistical significance（χ2=0.72,P0.05;χ2=0.98,P0.05）.③ Snail protein and mRNA expression was not related to age and tumor size（P0.05）. Conclusion The expression of Snail protein and mRNA is positive correlated,and closely related to the genesis and development of breast carcinoma and lymphatic metatasis,so it may be considered as a biological marker of progresses and metastasis of breast cancer.