A Bioluminescent γ-Aminobutyrate Assay for Monitoring Its Release from Inhibitory Nerve Endings |
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| Authors: | M. Israë l, B. Lesbats |
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| Affiliation: | Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif sur Yvette, France |
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| Abstract: | Abstract: A bioluminescent GABA assay is described. The principle of the procedure is based on the action of GABASE (GABA-aminotransferase plus succinic semialdehyde dehydrogenase), coupled to the detection of succinic semialdehyde and NADH, using Photobacterium luciferase. The method was used for monitoring GABA release from depolarized brain slices. |
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| Keywords: | GABA release Bioluminescent GABA assay GABASE |
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