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Depolarization of the membrane potential by beta-lactams as a signal to induce autolysis.
Authors:András Penyige  János Matkó  Eleonóra Deák  Andrea Bodnár  Gy?rgy Barabás
Affiliation:University of Debrecen, Medical and Health Science Center, Debrecen, H-4012, Hungary. penyige@jaguar.dote.hu
Abstract:The effect of beta-lactam antibiotics that are known to inhibit cell wall biosynthesis and induce cell wall autolysis on the electrophysiological state of the plasma membrane in Streptomyces griseus was studied. Addition of various beta-lactam antibiotics induced a dose- and growth-stage-dependent depolarization of the membrane potential of Streptomyces griseus. The hydrolyzed biologically inactive derivative penicilloic acid had no depolarizing effect on the membrane potential. The ionophore gramicidin D, while depolarizing the membrane potential, also induced a dose-dependent increase in cell wall lysis. These observations suggest that alteration of the transmembrane potential could be an important signal in triggering cell wall autolysis of S. griseus.
Keywords:mechanism of action of β-lactam antibiotics   effect of penicillin G, cephotaxime, gramicidin D   induction of cell wall autolysis   fluorescence assay of bacterial membrane potential   DiOC6(3)   protoplasts   Streptomyces griseus   kanamycin
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