| 肌苷产生菌B.Subtilis H841在2升罐中的发酵 |
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| 引用本文： | 王梦鹤,刘伟民,刘风元. 肌苷产生菌B.Subtilis H841在2升罐中的发酵[J]. 生物技术, 1991, 0(4) |
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| 作者姓名： | 王梦鹤 刘伟民 刘风元 |
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| 作者单位： | 黑龙江省科学院应用微生物研究所 哈尔滨(王梦鹤，刘伟民)，黑龙江省科学院应用微生物研究所 哈尔滨(刘风元) |
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| 摘 要： | 枯草芽孢杆菌(Bacillus Subtilis)H841肌苷产生菌是腺嘌呤、组氨酸、硫胺素三重缺陷型菌株,并对8—氮杂乌嘌呤、6—巯基嘌呤有抗性。在摇瓶中产肌胺18.1克/升,在2L自控发酵罐中最高可产肌苷19.6克/升,在流加葡萄糖情况下可产肌苷26.2克/升。控制pH较不控制pH发酵肌苷产量有较大的增加,控制pH发酵并补加营养时,肌苷产量可稳定地增长,但对葡萄糖的转化率是相同的。
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| 关 键 词： | 肌苷 发酵 枯草芽孢杆菌 |
Production of inosine in 2 litre fermentor by Bucillus Subtilis H841 |
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| Wang Menghe Liu weimin Liu Fengyuan. Production of inosine in 2 litre fermentor by Bucillus Subtilis H841[J]. Biotechnology, 1991, 0(4) |
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| Authors: | Wang Menghe Liu weimin Liu Fengyuan |
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| Abstract: | Production of inosine was studied by a mutant strain of Bacillus subtilis H841 which is adtnine, histidine and thiamine auxotroph and is 8-azaquan -ine, 6- mercaptopurine resistant mutant. The production of inosine was 18.1 g/l in shake flasks. H841 accumulated 19.6 g/1 of Inosine in 2 litre fermentor and the strain accumulated inosine was 26.2 g/l.The production of inosine was more pH control than pH no control, controlling pH and feeding accumulated inosine was higher but glucose conversion rate was Small |
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| Keywords: | inosine fermentation Bacillus subtilis |
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