Persistent infection with bovine herpesvirus-1 (infectious bovine rhinotracheitis virus) in cultured hamster cells |
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Authors: | F J Michalski G D Hsiung |
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Institution: | (1) Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut;(2) Virology Laboratory/151B, Veterans Administration Hospital, 06516 West Haven, Conn |
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Abstract: | Summary Bovine herpesvirus-1 infection in hamster embryo cells was found to be dependent upon input multiplicity; productive infection
was achieved at input multiplicities greater than one, while persistent infection was established when input multiplicities
were about 0.5. This persistence was characterized by a noncyclic, minimal degree of cytopathic effect with a low level of
released virus. Maintenance of the persistently infected cultures did not require external supportive measures. Subcultivation
of the persistently infected cultures led to virus replication followed by CPE and then cell regrowth. Within 3 to 4 weeks
after subcultivation a persistent infection was re-established. The possible mechanism for the bovine herpesvirus persistence
in hamster cells is discussed.
This work was supported in part by Public Health Service Research Contract FDA 233-74-1035 and by Research Grant AI-08648
from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. |
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Keywords: | viral persistence bovine herpesvirus hamster embryo cells |
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