醋酸棉酚对大鼠睾丸HCG反应性及其受体功能的影响

给大鼠灌服醋酸棉酚30 mg/kg/d,每周6次,持续4周。给药2周后,血浆睾酮水平显著下降并持续至第4周,同时间质细胞呈萎缩性改变。醋酸棉酚明显抑制成年大鼠对HCG反应性,使睾丸LH/HCG受体亲和力下降,受体数目略有减少。结果提示,醋酸棉酚抑制大鼠睾丸酮的产生及降低成年大鼠睾丸对HCG的反应性,推测其机制是由于醋酸棉酚干扰了睾丸HCG受体功能而造成的。

EFFECT OF GOSSYPOL ACETATE ON THE TESTICULAR HCG REACTIVITY AND IT'S RECEPTOR IN THE RATS

20 male rats were used in this experi-ment.The rats were fed with gossypol acetate at the dose of 30 mg/kg/day,six times per week orally for 4 weeks.Plasma testosterone was determined by RIA.The number of tes-ticular LH/HCG receptor and receptor affini-ty were determined by RRBA.Results showed that plasma testosterone levels were decreased significantly after 2,3 and 4 weeks of admi-nistration of gossypol.The interstitial cellsbecame atrophy morphologically.The forma-tion of testosterone of rats treated with gossy-pol in response to HCG was reduced signi-ficantly after HCG injection for 120 min.The number of testicular LH/HCG receptor was not altered significantly but its affinity was obviously reduced.These results suggest that gossypol inhibits the production of testosterone and HCG reactivity of testis through interfe-ring the function of testicular HCG receptors.