Liquid chromatography determination of 10-hydroxycamptothecin in human serum by a column-switching system containing a pre-column with restricted access media and its application to a clinical pharmacokinetic study |
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Authors: | Ma Jun Jia Zheng-Ping Zhang Qiang Fan Jun-Jie Jiang Ning-Xi Wang Rong Xie Hua Wang Juan |
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Affiliation: | Department of Pharmacy, General Hospital of Lanzhou Command of PLA, Lanzhou 730050, China. mb1642@public.lz.gs.cn |
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Abstract: | A simple, rapid, sensitive column-switching HPLC method is described for the analysis of the 10-hydroxycamptothecin (HCPT) in human serum. A pre-column containing restricted access media (RAM) is used for the sample clean-up and trace enrichment and is combined with a C18 column for the final separation. The analytical time is 8 min. The HCPT is monitored with fluorescence detector, excitation and emission wavelengths being 385 and 539 nm, respectively. There is a linear response range of 1-1000 ng/ml with correlation coefficient of 0.998 while the limit of quantification is 0.1 ng/ml. The intra-day and inter-day variations are less than 5%. This analytic procedure has been applied to a pharmacokinetic study of HCPT in clinical patients and the pharmacokinetic parameters of one-compartment model are calculated. |
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Keywords: | Pharmacokinetics Restricted access media 10-Hydroxycamptothecin |
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