In vivo biosensors: mechanisms,development, and applications |
| |
Authors: | Shuobo Shi Ee Lui Ang Huimin Zhao |
| |
Institution: | 1.Metabolic Engineering Research Laboratory, Science and Engineering Institutes,Agency for Science, Technology and Research,Singapore,Singapore;2.Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology,Beijing,People’s Republic of China;3.Department of Chemical and Biomolecular Engineering,University of Illinois at Urbana-Champaign,Urbana,USA |
| |
Abstract: | In vivo biosensors can recognize and respond to specific cellular stimuli. In recent years, biosensors have been increasingly used in metabolic engineering and synthetic biology, because they can be implemented in synthetic circuits to control the expression of reporter genes in response to specific cellular stimuli, such as a certain metabolite or a change in pH. There are many types of natural sensing devices, which can be generally divided into two main categories: protein-based and nucleic acid-based. Both can be obtained either by directly mining from natural genetic components or by engineering the existing genetic components for novel specificity or improved characteristics. A wide range of new technologies have enabled rapid engineering and discovery of new biosensors, which are paving the way for a new era of biotechnological progress. Here, we review recent advances in the design, optimization, and applications of in vivo biosensors in the field of metabolic engineering and synthetic biology. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|