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Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli |
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| Authors: | Yasunobu Kano Setsuo Yoshino Morimasa Wada Kazushige Yokoyama Masahiro Nobuhara and Fumio Imamoto |
| Institution: | (1) Laboratory of Molecular Genetics, (Riken) The Institute of Physical and Chemical Research, Hirosawa, 2-1, 351-01 Wako, Saitama;(2) Central Research Laboratories, Mitsui Toatsu Chemicals, Inc., Kasama-cho, 1190, Totsuka-ku, 247 Yokohama, Kanagawa;(3) Research Laboratories of Cell Science, Mochida Pharmaceutical Co., LTD, 1-1, 1-chome, Kamiya, Kita-ku, 115 Tokyo, Japan |
| Abstract: | Summary The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream from the translational initiation codon (GUG) of the HU-1 gene. |
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