The differential activation of ornithine decarboxylase in synchronized cultures maintained with a salts/glucose medium |
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Authors: | Marco Meloni Maddalena Perra Max Costa |
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Institution: | Department of Pharmacology and Toxicology, College of Medicine, Texas A & M University, College Station, TX 77843, USA |
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Abstract: | Asparagine and cAMP caused differential activation of ornithine decarboxylase activity that was dependent upon the cell cycle phase of Chinese Hamster Ovary cells maintained with a salts/glucose medium. Prior to incubation in the salts/glucose medium, stationary phase cultures were stimulated to proliferate by refeeding with fresh complete growth medium. Induction of ornithine decarboxylase activity was dependent upon two conditions: (1) the salts/ glucose medium required supplementation with an appropriate amino acid and cAMP; (2) cultures had to be in late G1/early S phase when placed in the salts/glucose medium. Cultures in G0 phase had moderate capacity for ornithine decarboxylase enhancement but lost this capacity as they traversed into early G1 or into mid S phase. These results demonstrate the importance of the cell cycle in modulating the activation of ornithine decarboxylase by asparagine and cAMP. |
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