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Aqueous two-phase extraction for downstream processing of amyloglucosidase |
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| Authors: | S Tanuja ND Srinivas KSMS Raghava Rao MK Gowthaman |
| Institution: | a Department of Food Engineering, Central Food Technological Research Institute, Mysore 570013, India b Department of Fermentation Technology, Central Food Technological Research Institute, Mysore 570013, India |
| Abstract: | A polymer/salt aqueous two-phase system has been successfully employed for separation and purification of amyloglucosidase. The effects of system pH, molecular weight of polymer and composition of the two-phase system on amyloglucosidase partition behaviour in polyethylene glycol (PEG 4000, 6000)/disodium hydrogen phosphate were investigated. Experimental data are explained based on Kim's theoretical model for the prediction of biomolecule partitioning in a PEG/salt system. |
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