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Identification of cytochromes o and a3 as functional terminal oxidases in the thermophilic bacterium, PS3
Authors:Robert K. Poole   Robert I. Scott   Baldev S. Baines   Ian Salmon  David Lloyd
Affiliation:1. Department of Microbiology, Queen Elizabeth College (University of London), Campden Hill, London W8 7AH, England;2. Department of Microbiology, University College, Newport Road, Cardiff DF2 1TA, Wales England
Abstract:Low-temperature photodissociation spectra of membranes from the thermophile PS3 reveal cytochromes o and a3. The latter reacts with O2 at −103°C to give a light-insensitive compound(s), but the initial stages of O2 binding to cytochrome o could not be studied under these conditions. Photochemical action spectra identify cytochromes a3 and o, but not a CO-binding c-type cytochrome, as functional terminal oxidases in this bacterium.
Keywords:Cytochrome oxidases   Bacterial respiration   Cytochrome o   Cytochrome aa3
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