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Study of markers of human erythroid differentiation in hybrid cells
Authors:Albert Deisseroth  Arthur Nienhuis
Institution:(1) Children’s Hospital Medical Center, 02115 Boston, Massachusetts;(2) the Molecular Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 20014 Bethesda, Maryland
Abstract:Summary Somatic cell hybrids exhibiting co-expression of the globin genes of two species were generated by fusion of mouse erythroleukemia cells with Chinese hamster or human marrow erythroid cells. In contrast, extinction of the mouse globin genes occurred in hybrids formed between the erythroleukemia cells and human fibroblasts. Direct detection of the human globin genes in human X mouse fibroblast hybrids was achieved by annealing of DNA from these cells to human globin complementary DNA. This method was developed to permit the chromosomal assignment of the human globin genes. Presented in the formal symposium on Somatic Cell Genetics at the 27th Annual Meeting of the Tissue Culture Association, Philadelphia, Pennsylvania, June 7–10, 1976. Some of this work was conducted during the tenure of a National Research Fellowship 1 F32 AM05080-01 held by A.D.
Keywords:globin gene  chromosomal localization  regulation  gene expression
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