| 马兜铃酸对斑马鱼肾损伤因子（KIM-1）表达的影响 |
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| 引用本文： | 王雪,韩利文,何秋霞,孙晨,张云,王希敏,刘可春.马兜铃酸对斑马鱼肾损伤因子（KIM-1）表达的影响[J].动物学杂志,2018,53(4):622-627. |
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| 作者姓名： | 王雪 韩利文 何秋霞 孙晨 张云 王希敏 刘可春 |
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| 作者单位： | 齐鲁工业大学(山东省科学院)山东省科学院生物研究所山东省科学院药物筛选技术重点实验室山东省生物检测技术工程实验室 |
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| 基金项目： | 山东省自主创新重大专项（No. 2014ZZCX0215） |
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| 摘 要： | 采用分子定量方法检测斑马鱼(Danio rerio)幼鱼组织中KIM-1蛋白含量和基因表达水平,了解马兜铃酸处理后斑马鱼体内肾损伤因子的表达变化情况,并与幼鱼表型变化相比较,探讨KIM-1作为肾功能特异标志物在斑马鱼肾功能损伤评价中的应用价值。结果显示,马兜铃酸处理30 h后,幼鱼出现水肿,水肿发生率呈剂量依赖性特点。0.5~5.0μmol/L马兜铃酸处理组幼鱼水肿发生率与对照组差异不明显,但幼鱼KIM-1因子的蛋白含量及Kim-1基因表达水平比正常对照组显著升高,在马兜铃酸浓度为2μmol/L时达到最高。表明在肾功能损伤检测中,KIM-1是一种比表型更早发生变化的指标。
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| 关 键 词： | 斑马鱼 肾损伤因子（KIM-1） 马兜铃酸 肾毒性 生物标志物 |
| 收稿时间： | 2017/9/7 0:00:00 |
| 修稿时间： | 2018/5/31 0:00:00 |
Effect of Aristolochic Acid on Expression of KIM-1 in Zebrafish |
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| Institution: | Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan 250301,Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan 250301,Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan 250301,Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan 250301,Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan 250301,Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan 250301 and Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Jinan 250301 |
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| Abstract: | |
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| Keywords: | Zebrafish Danio rerio Kidney injury molecule (KIM-1) Aristolochic acid Nephrotoxicity Biomarker |
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