Phenotypical and structural characterization of the Arabidopsis mutant involved in shoot apical meristem |
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Authors: | Zhe Hu Ping Li Jinfang Ma Yunlong Wang Xinyu Wang and Chongying Wang |
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Institution: | (1) Institute of Cell Biology, School of Life Science, Lanzhou University, Lanzhou, 730000, China |
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Abstract: | An Arabidopsis mutant induced by T-DNA insertion was studied with respect to its phenotype, microstructure of shoot apical meristem (SAM)
and histochemical localization of the GUS gene in comparison with the wild type. Phenotypical observation found that the mutant
exhibited a dwarf phenotype with smaller organs (such as smaller leaves, shorter petioles), and slower development and flowering
time compared to the wild type. Optical microscopic analysis of the mutant showed that it had a smaller and more flattened
SAM, with reduced cell layers and a shortened distance between two leaf primordia compared with the wild type. In addition,
analysis of the histo-chemical localization of the GUS gene revealed that it was specifically expressed in the SAM and the
vascular tissue of the mutant, which suggests that the gene trapped by T-DNA may function, in the SAM, and T-DNA insertion
could influence the functional activity of the related gene in the mutant, leading to alterations in the SAM and a series
of phenotypes in the mutant.
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Translated from Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(2): 228–232 译自: 西北植物学报] |
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Keywords: | Arabidopsis shoot apical meristem mutant GUS T-DNA |
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