芦笋丛生芽增殖培养技术

本试验以芦笋新品种"津宁"为材料,探讨植物生长调节剂对不同外植体产生丛生芽的效应。试验结果表明,NAA、6-BA和KT三者配合使用有利于丛生芽的增殖:以茎尖为增殖外植体时,培养基用MS+NAA0.1 mg/L+6-BA0.2~0.3 mg/L+KT 0.1 mg/L,30 d时平均每瓶可以产生20.5个丛生芽;以茎中段为外植体时,培养基用MS+NAA 0.1 mg/L+6-BA 0.3~0.4 mg/L+KT 0.1 mg/L,30 d时平均每瓶可产生33.5个丛生芽;以茎基为外植体时,培养基用MS+NAA 0.1 mg/L+6-BA 0.3~0.5 mg/L+KT 0.1 mg/L,30 d时平均每瓶可以产生43.1个丛生芽。进行试管苗工厂化生产时,增殖外植体以茎基为最佳。

Study on Enrichment Culture of Asparagus officinalis Cespitose Buds

Effects of plant growth regulators on multiplication of cespitose buds from Asparagus officinalis new species ‘Jinling’ were studied.The result indicated that the admixture of NAA, 6-BA and KT gave good result for multiplication of cespitose buds.Taking the stem apex as explant, after 30 d, there were 26.3 cespitose buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.2~0.3 mg/L+KT 0.1 mg/L;taking the mid stem as explant, after 30 d, there were 33.5 buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.3~0.4 mg/L+KT 0.1 mg/L;taking the stem base as explant, there were 43.1 buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.3~0.5 mg/L+KT 0.1 mg/L.The stem base was the optimum explant for rapid propagated.